7061 spores  (ATCC)

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

Article Snippet: We performed spore decoating by using detergent in the presence of salt and a reducing agent under alkaline conditions to extract the coat proteins of SAFR-032 and ATCC 7061 spores.

Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

Article Snippet: We performed spore decoating by using detergent in the presence of salt and a reducing agent under alkaline conditions to extract the coat proteins of SAFR-032 and ATCC 7061 spores.

Techniques: SDS Page

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

Article Snippet: To verify the genomic information, we investigated SAFR-032 and ATCC 7061 spore protein extracts.

Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

Article Snippet: To verify the genomic information, we investigated SAFR-032 and ATCC 7061 spore protein extracts.

Techniques: SDS Page

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of proteins extracted from intact and decoated B. pumilus spores. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 1A and 2A, intact spore protein extracts; 1B and 2B, decoated spore protein extracts; 1C and 2C, coat protein extracts. Lanes 1A and 1B were cut into ∼1.5-mm slices from the bottom to the top for protein identification by LC-MS/MS. A similar amount of protein (∼10 μg) was loaded into each well. The black arrowheads indicate a sporulation-related manganese catalase (YjqC). The white arrowheads indicate the second manganese catalase (BPUM_1305). The numbers indicate the excised PAGE bands.

Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).

Techniques: SDS Page, Liquid Chromatography with Mass Spectroscopy

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: SDS-PAGE analysis of B. pumilus spore protein extracts. Lanes: ST, molecular mass standards (sizes in kDa are on the left); 1, SAFR-032; 2, ATCC 7061; 3, BG-B79; 4, 8A4; 5, 8A6; 6, 14A1. A similar amount of protein was loaded into each 12% SDS-PAGE well. The rectangles (∼1 cm) were excised, digested in the gel with trypsin, and sequenced. The arrowheads indicate the sporulation-related manganese catalase (YjqC). BPUM_1305 was identified in all of the gel extracts tested.

Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).

Techniques: SDS Page

Journal: Applied and Environmental Microbiology

Article Title: Protection of Bacillus pumilus Spores by Catalases

doi: 10.1128/AEM.01211-12

Figure Lengend Snippet: Catalase specific activities detected in B. pumilus spore suspensions

Article Snippet: Catalase activity optimization was performed at various pHs and temperatures for SAFR-032 and ATCC 7061 spore suspensions (data not shown).

Techniques: Activity Assay